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Non-normalized cDNA libraries

21 libraries from clone log50 cDNA are constructed and arrayed in 384-well plates. The clones are stored in freezing buffer. These libraries have not been normalized, except for Contl Exposure Norm.

Library

Condition

Stage

# of Arrayed Plates

Plate IDs

1

Hypoxia

Adult

6

c_Log50AdHypoxc031804001-006

2

Hypoxia

Juvenile

6

c_Log50ImHypoxc031804001-006

3

Short UV

Mixed

6

c_Log50MxSuvexpo040104001-006

4

Long UV

Mixed

6

c_Log50MxLuvexpo040104001-006

5

Unchallenged

Juvenile

4

c_Log50ImUnchal030504001-004

6

Low Cd

Mixed

7

c_Log50Mx_LowCd071904001-007

7

Low As

Mixed

7

c_Log50Mx_LowAs071904001-007

8

Low Zn

Mixed

7

c_Log50Mx_LowZn071904001-007

9

Mixed Metals

Mixed

7

c_Log50Mx_Mxmet071904001-007

10

Contl Exposure

Mixed

10

c_Log50Mx_Dcctl062204001-010

11

Contl Exposure Norm

Mixed

10

c_Log50Mx_DcctlN062204001-010

12

Contl Exposure

Mixed

13

c_Log50Mx_Dcctl121803001-013

13

Chaoborus

Adult

12

c_Log50AdChaobs090804001-12

14

Starvation

Juvenile

6

c_Log50ImStarve090804001-006

15

Starvation

Adult

6

c_Log50AdStarve090804001-006

16

Microcystis

Juvenile

6

c_Log50ImMccyst090804001-006

17

Microcystis

Adult

6

c_Log50AdMccyst090804002-007

18

Fish

Juvenile

6

c_Log50Im_Fish_090804001-006

19

Fish

Adult

6

c_Log50Ad_Fish_090804001-006

20

Methyl Farnesoate /males

Juvenile

6

c_Log50ImMfmale090804001-006

21

Methyl Farnesoate /males

Adult

6

c_Log50AdMfmale090804001-006

Details on cDNA library construction

PCR based analysis of cDNA inserts

E. Bohuski (CGB) produced crude plasmid preps from one 384-well plate of each library. The plasmids are digested with restriction enzymes to release the cDNA inserts. The products are electrophoresed on agarose gels and visualized against size standards. Click for the brief ccp protocol or download the detailed protocol. The goal is to identify libraries with reduced insert sizes due to poor cDNA synthesis.

Synonym table

  • CANA = Adult starvation
  • CANB = Juvenile starvation
  • CANC = Juvenile unchallenged
  • CANF = Mixed stage control
  • CANG = Mixed stage control
  • CANH = Mixed stage control Normalized
  • CANI = Adult fish exposure
  • CANN = Juvenile fish exposure
  • CANO = Adult hypoxia
  • CANP = Juvenile hypoxia
  • CANS = Mixed stage short UV
  • CANT = Mixed stage long UV
  • CANU = Mixed stage arsenic
  • CANW = Mixed stage cadmium
  • CANX = Mixed stage zinc
  • CANY = Adult methyl farnesoate/males
  • CANZ = Juvenile methyl farnesoate/males
  • CAOA = Adult microcystis
  • CAOB = Juvenile microcystis
  • CAOC = Mixed stage mixed metals
  • CAOF = Adult chaoborus exposure
  • CUCS = Adult starvation
  • CUCW = Juvenile starvation
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